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KMID : 0370219960400020212
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Yakhak Hoeji
1996 Volume.40 No. 2 p.212 ~ p.217
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Improvement of Carbapenem Antibiotics Productivity in S. cattleya by Transformation
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박지선/Park JS
이강만/Lee KM
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Abstract
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Streptomyces cattleya is a producer of carbapenem antibiotics, thienamycin and N-acetylthienamycin, which have potent and broad-spectrum antibacterial activities. We studied on strain improvement for antibiotic productivity of S. cattleya by transformation technique which employed S.cattleya protoplasts and chromosomal DNAs of glutamic acid producers: Corynebacterium glutamicum and Arthrobacter simplex. 150 Transformant strains were cultured and bioassayed using Bacillus subtilis and Staphylococcus aureus as test organisms. 8.7% of transformants tested showed 1.4~2.6 fold higher productivities than wild type which produced 1.61 +/- 0.67mcg/ml. The best transformant produced 8.36 +/- 2.84mcg/ml carbapenems.
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